FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831627

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831627
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12608633
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC138950.065.87628120
GAGCACA210300.045.207214120
AGAGCAC283850.032.910004118-119
AAGAGCA437650.021.58837120
GAAGAGC494550.019.938625120
GGAAGAG639050.016.219091120
TCGGAAG713050.014.673046118-119
CGGAAGA756500.014.22031120
ATCGGAA846350.012.423836118-119
GATCGGA864750.012.319056120
AGATCGG943450.011.569036120
TAGATCG172900.010.378437118-119
CTAGATC47850.010.041212114-115
AAGATCG455650.08.72274114-115
TTAGATC72200.08.510819114-115
CACACGT34450.07.982529120
CCTAGAT16550.07.9124765120
GAGATCG344450.07.907653120
GCCTAGA9106.211849E-97.542798118-119
CAGATCG353750.07.5211205118-119
Produced by FastQC (version 0.11.5)