FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831629

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831629
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14189261
Sequences flagged as poor quality0
Sequence length50-126
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC123250.073.045044120
GAGCACA199650.046.910927120
AGAGCAC272950.035.571663120
AAGAGCA469050.021.694817120
GAAGAGC489350.021.059738120
GGAAGAG593100.017.262075118-119
CGGAAGA679400.016.017715120
TCGGAAG698650.015.010471120
ATCGGAA818950.013.494055120
GATCGGA846150.012.90708120
AGATCGG943500.011.632686118-119
TAGATCG242850.010.892443116-117
AAGATCG388050.09.383411118-119
CTAGATC56250.09.02583116-117
GAGATCG307700.08.785953114-115
CACGTCT35000.08.14817120
CAGATCG287850.08.054953118-119
TAAGATC157200.07.9610157114-115
TTAGATC123850.07.8070188116-117
CACACGT28300.07.3289037120
Produced by FastQC (version 0.11.5)