FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831630

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831630
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14435861
Sequences flagged as poor quality0
Sequence length55-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCTAG20600.026.736721120
TCTAGGT18500.021.619925120
CTAGGTG12450.020.426617114-115
GTCTAGG14500.019.487461114-115
CGTCTAG20700.019.322155120
TCTAGGG15750.018.531815116-117
CTAGGGA11050.017.78237108-109
GTCTAGA21650.014.936887114-115
AAAAAAA1412250.014.397472120
GATCTAG19150.014.099718112-113
ATCTAGG19950.012.7474375112-113
CTAGGGC5608.913048E-1110.88235116-117
CTCAGTG37250.010.561389120
CTAGGGT7200.010.245955116-117
GAGTCTA32200.09.945465112-113
CGTATCA626700.09.782457120
CGTAGTG53000.09.526009120
GTATCAT622050.09.3285475120
GCGTCGG99750.09.268348120
TCATTAA490500.09.223721120
Produced by FastQC (version 0.11.5)