FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14435861
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC184350.088.10228120
GAGCACA296800.056.607758120
AGAGCAC396200.041.68337118-119
AAGAGCA598850.027.60973118-119
GAAGAGC668700.024.725536116-117
GGAAGAG813800.020.28877116-117
CGGAAGA963150.017.257864114-115
TCGGAAG978000.016.733625114-115
ATCGGAA1147200.014.495189112-113
GATCGGA1158050.014.1875305112-113
AGATCGG1261550.013.178275110-111
CACACGT46750.012.296428120
TAGATCG306250.012.159339110-111
CTAGATC56800.011.589168108-109
ACACGTC50350.010.656091120
CACGTCT50800.010.561695120
GAGATCG506400.010.527631110-111
TTAGATC100700.010.033927118-119
AAGATCG371850.09.852795120
CAGATCG456300.09.574668120
Produced by FastQC (version 0.11.5)