FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12270280
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC129150.060.715088120
GAGCACA188100.043.40409120
AGAGCAC245150.032.960804120
AAGAGCA392100.022.123383120
GAAGAGC438450.019.195381120
GGAAGAG546400.016.632433120
TCGGAAG639400.014.314348118-119
CGGAAGA648150.014.150937120
ATCGGAA769250.012.838443120
GATCGGA770900.012.299922118-119
AGATCGG843250.011.405457118-119
TAGATCG196850.011.156189120
AAGATCG412150.010.076925120
CTAGATC51850.09.716819116-117
TTAGATC89100.08.844117120
GAGATCG286350.08.334806116-117
TAAGATC162250.07.5240464118-119
CCTAGAT17200.07.511703112-113
ACACGTC47750.07.169269120
CAGATCG268000.06.9893622118-119
Produced by FastQC (version 0.11.5)