FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15187359
Sequences flagged as poor quality0
Sequence length88-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA1367250.011.6202135120
CGTATCA533650.09.169229120
GCGTCGG116100.09.060887120
GGTCGCC835550.09.036122120
TGGTCGC877550.09.014651120
GGTGGTC1027600.08.663549120
GTGGTCG955600.08.649508120
GCCGTAT671800.08.554266120
CGCCGTA719050.08.477031120
TCATTAA456100.08.474534120
TCGGTGG1210650.08.470818120
CGGTGGT1135100.08.383236120
GTCGCCG849900.08.147972120
GTATCAT540650.08.1054325120
CCGTATC622650.08.090305120
ATCTCGG1494050.07.8900065120
ATCATTA524700.07.7675405120
CTCGGTG1311100.07.757636120
TCGCCGT820650.07.6546125120
TCTCGGT1395650.07.6193194120
Produced by FastQC (version 0.11.5)