FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13022723
Sequences flagged as poor quality0
Sequence length51-126
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT56250.030.5074634
TCCGATC61750.028.1788753
CGATCTA17000.027.1775995
CTTCCGA96450.019.0360491
TCTCGGG23300.014.166071120
TTCCGAT136750.013.4700332
CGGTGGG19050.013.231131120
AAAAAAA2437200.013.011108120
GCGTCGG62600.012.750288120
GTATCAT890400.010.473903120
CCGTATC1019050.010.211658120
CGTATCA928250.010.189055120
CGATCTC55650.010.0273715
GCCGTAT1110050.09.56915120
CGCCGTA1201250.09.32725120
TCGCCGT1332000.09.110025120
TGGTCGC1621700.09.014662120
GTCGCCG1430150.09.009343120
TCATTAA814750.08.949413120
GGTCGCC1504450.08.9433565120
Produced by FastQC (version 0.11.5)