FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13022723
Sequences flagged as poor quality0
Sequence length50-126
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC208300.0139.04489120
GAGCACA358800.080.94795120
AGAGCAC496600.058.51151118-119
AAGAGCA737650.038.76036118-119
GAAGAGC829650.035.041267116-117
GGAAGAG982300.029.11192116-117
CCGATCT61500.027.4886914
TCCGATC64300.026.6840863
CGATCTA17450.026.6058275
CGGAAGA1158900.025.12948114-115
TCGGAAG1231150.023.223476114-115
CACACGT47450.020.930132120
ATCGGAA1414300.020.698126112-113
CACGTCT54150.020.586206120
GATCGGA1459000.019.648083112-113
ACACGTC54550.019.50638120
AGATCGG1564950.018.627928110-111
CTTCCGA95250.017.990851
TAGATCG424700.016.52683114-115
CGTCTGA78050.016.359867120
Produced by FastQC (version 0.11.5)