FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831644

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831644
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15891613
Sequences flagged as poor quality0
Sequence length65-126
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCAT153000.017.1027261
CCTTCAC88550.016.5375651
CATTACC113700.014.6742061
GCGTCGG87800.014.506511120
CCTTGAT118800.014.3473651
CCTACAC37500.014.2439051
CCTTTAT200700.013.2771811
CAATACC137700.013.2498191
CCTGCAT118150.013.1055811
CCTCCAT92150.012.9606881
GATTACC75400.012.7355851
CCTACAT58050.012.5098891
CCTGCAC96800.012.4620931
CCTCCAC69000.012.090271
CCTTTAC111700.011.8206071
CCTCGAT66500.011.2812871
CAACACC121000.011.1104841
CCCCCCC4450.001572609410.7894741
CATTGAT166600.010.7712591
CATGCAC50250.010.5103441
Produced by FastQC (version 0.11.5)