FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831645

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831645
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15891613
Sequences flagged as poor quality0
Sequence length50-126
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC224800.093.22457120
GAGCACA378500.057.651077120
AGAGCAC517950.041.374134118-119
AAGAGCA779600.027.558342118-119
GAAGAGC869000.025.354757120
GGAAGAG1043050.020.979193118-119
CGGAAGA1201350.018.620817120
TCGGAAG1296550.017.434296120
CACGTCT60550.016.930544120
CACACGT57550.015.650091120
ATCGGAA1510850.015.133349118-119
CCTTCAC93200.014.72343351
CCTTCAT157900.014.17542651
ACACGTC65950.014.100871120
GATCGGA1608400.014.024878118-119
ACGTCTG67600.013.648375120
CATTACC112650.013.5791611
AGATCGG1783950.012.947761116-117
CCTTGAT124350.012.07537651
CCTACAT59650.012.0679841
Produced by FastQC (version 0.11.5)