FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13644717
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC175300.062.31922120
GAGCACA264200.040.53381120
AGAGCAC344100.033.490253120
AAGAGCA512500.022.84068120
GAAGAGC576750.020.098259118-119
GGAAGAG675600.017.102795118-119
CGGAAGA803850.015.6933565120
TCGGAAG811300.014.868578118-119
ATCGGAA956800.012.952385120
GATCGGA980000.012.790084120
ACACGTC73000.012.640108120
AGATCGG1097050.011.720134120
CACACGT69400.011.160701120
TAGATCG214000.011.109999120
CTAGATC60200.010.622825118-119
CACGTCT68750.010.1885805120
TTAGATC77150.09.903138118-119
ACGTCTG84050.09.295513120
AAGATCG428200.09.172811114-115
GAGATCG388500.08.876302120
Produced by FastQC (version 0.11.5)