FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831648

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831648
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13025240
Sequences flagged as poor quality0
Sequence length54-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG11350.016.73566120
AGTCTAG10200.013.485275120
TCTAGGT10803.092282E-1111.523132120
TCTAGGG8850.010.50186116-117
GTCTAGG8400.010.459683114-115
AGATCTC267050.08.829795120
GTGTAGA366850.08.7487955120
TAGATCT266700.08.668876118-119
AGAGTGT422900.08.487575120
GAGTGTA386900.08.481637120
ATCTCGG250500.08.471845120
GATCTCG282950.08.194723120
GTGGTCG169550.08.189903120
GTAGATC365550.08.116937120
AGTGTAG361100.08.053714120
TCTCGGT227500.07.831198120
AAGAGTG474200.07.7074966120
GGTGGTC190900.07.582756120
CTCGGTG224850.07.544797120
TCGGTGG223250.07.5401917120
Produced by FastQC (version 0.11.5)