FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831649

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831649
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13025240
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC123500.058.840137120
GAGCACA187150.042.766155120
AGAGCAC247200.031.85892120
AAGAGCA384450.021.518599120
GAAGAGC435600.018.433838118-119
GGAAGAG532700.015.674342120
TCGGAAG617500.014.44541120
CGGAAGA643200.013.698855120
GATCGGA728850.012.897916120
ATCGGAA724200.012.272853120
TAGATCG197250.012.052704120
AGATCGG821300.011.539685120
CTAGATC50400.010.47747118-119
TTAGATC74400.09.818825120
AAGATCG283050.09.05575120
CCTAGAT18050.08.68786114-115
GAGATCG355300.08.341013118-119
CAGATCG327900.07.8561945120
CCCTAGA8601.0816015E-57.4512506120
GCTAGAT20950.07.179829116-117
Produced by FastQC (version 0.11.5)