FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12957995
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC108650.060.983284120
GAGCACA161450.043.224606120
AGAGCAC219350.032.136623120
AAGAGCA371800.020.546724120
GAAGAGC393750.018.793018118-119
GGAAGAG499800.014.728479118-119
TCGGAAG554000.014.101903120
CGGAAGA580900.012.914909116-117
GATCGGA661900.011.7546215120
ATCGGAA668250.011.433007118-119
AGATCGG708500.011.026753120
TAGATCG194300.010.621955116-117
CTAGATC50150.09.464572120
TTAGATC88550.08.613502116-117
AAGATCG303750.08.0454235120
GAGATCG253800.07.5509696118-119
CAGATCG267250.07.0511975114-115
GCTAGAT20850.06.6110864108-109
CCTAGAT18250.06.3218307114-115
GCCTAGA8354.6497524E-56.1425085106-107
Produced by FastQC (version 0.11.5)