FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831657

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831657
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14778665
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC120200.060.230637120
GAGCACA175550.039.462948120
AGAGCAC232150.031.267824120
AAGAGCA402450.019.033346120
GAAGAGC418450.018.381664120
GGAAGAG533450.014.505997118-119
TCGGAAG587800.013.454063120
CGGAAGA624000.012.847026120
ATCGGAA721250.011.865195120
GATCGGA719400.011.656732120
AGATCGG764350.010.980408118-119
TAGATCG207450.010.405135120
CTAGATC49150.08.728856116-117
GAGATCG302250.07.836788120
TTAGATC102550.07.823415120
AAGATCG337000.07.7514734118-119
CAGATCG280300.06.473812116-117
ATAGATC124500.05.8963623114-115
CCTAGAT22003.6379788E-125.344305110-111
GCTAGAT25800.05.296516114-115
Produced by FastQC (version 0.11.5)