FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11128048
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC121350.063.666725120
GAGCACA181650.042.88982120
AGAGCAC244400.033.28692120
AAGAGCA373150.021.56591118-119
GAAGAGC428250.020.75674120
GGAAGAG519850.016.28002118-119
CGGAAGA627500.014.580062120
TCGGAAG616250.014.319019120
ATCGGAA727050.012.780334120
GATCGGA726350.012.756868120
AGATCGG818900.011.743601120
TAGATCG180700.010.949251118-119
CTAGATC44600.010.926378116-117
TTAGATC70150.09.493812114-115
CACGTCT40000.09.421899120
GAGATCG341600.09.035375120
CACACGT37850.08.927049120
AAGATCG277250.08.776787118-119
ACACGTC45050.08.22149120
CAGATCG289550.08.179546118-119
Produced by FastQC (version 0.11.5)