FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19614543
Sequences flagged as poor quality0
Sequence length56-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCT137150.09.274001120
AGAGTGT235750.08.194666120
GAGTGTA210650.08.145761120
GTAGGGA380300.07.9511747120
GTAGATC204400.07.7784314120
TAGGGAA380850.07.7033916120
GTGTAGG455850.07.6073427120
AGTGTAG192650.07.427564118-119
GTCGTGT527450.07.370904120
TGTAGGG406850.07.2922087118-119
TCGTGTA504750.07.1080737120
CGTGTAG469350.07.0433917120
AGCGTCG664450.06.970791120
CGTCGTG596800.06.9567175120
GAGCGTC722650.06.8992214120
AAGAGTG282800.06.831303120
GTGTAGA216800.06.6555333118-119
GCGTCGT635400.06.6379685120
AGAGCGT765450.06.286148118-119
GAAGAGC912750.06.178785120
Produced by FastQC (version 0.11.5)