FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19614543
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC129550.036.17558120
GAGCACA168150.029.76574120
AGAGCAC206900.023.657984118-119
GAAGAGC382900.015.103518120
AAGAGCA366200.014.169566120
GGAAGAG486350.011.878299120
TCGGAAG506250.011.871222120
GATCGGA570700.011.31421120
CGGAAGA579400.010.848267120
ATCGGAA618500.010.568569120
AGATCGG637800.010.546522120
TAGATCG152150.09.663419120
CTAGATC37700.08.612435118-119
GAGATCG362500.06.954298118-119
TTAGATC69550.06.3419676116-117
GCTAGAT22000.06.265436118-119
CCTAGAT18250.06.042244116-117
CAGATCG310900.05.7537847120
AAGATCG281650.05.6117897120
TCTAGAT28952.5995178E-85.501945120
Produced by FastQC (version 0.11.5)