FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17466094
Sequences flagged as poor quality0
Sequence length50-126
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC271350.0186.2174120
GAGCACA452300.0107.8257120
AGAGCAC621600.079.69107118-119
AAGAGCA927250.052.97911118-119
GAAGAGC1005100.049.143364116-117
GGAAGAG1204600.040.674988116-117
ACACGTC72700.034.762238120
CGGAAGA1432250.034.368076114-115
CACGTCT74750.033.80889120
CACACGT66450.033.758583120
TCGGAAG1460150.033.308495114-115
ACGTCTG90250.030.204811120
ATCGGAA1720250.028.840355112-113
GCACACG75350.028.829048120
GATCGGA1712600.028.49326112-113
AGATCGG1843700.026.916956110-111
TAGATCG508100.024.815376110-111
GAGATCG526250.023.070627110-111
CAGATCG586300.022.303377110-111
CTAGATC104200.022.037962110-111
Produced by FastQC (version 0.11.5)