FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18399460
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC191600.068.34393120
GAGCACA294850.044.125008120
AGAGCAC402550.032.63284118-119
AAGAGCA642100.021.273628120
GAAGAGC692500.019.386011118-119
GGAAGAG845200.017.56029120
TCGGAAG1021400.014.441964120
CGGAAGA1018950.014.432066120
GATCGGA1198150.012.831931120
ATCGGAA1219050.012.452088120
AGATCGG1320300.011.581998118-119
TAGATCG293450.010.53605120
AAGATCG628150.09.916541120
CTAGATC73900.09.404686120
TTAGATC130050.09.021893114-115
GAGATCG436200.08.726034120
ACTAGAT31050.08.158456120
CCTAGAT24250.08.152977116-117
CAGATCG404150.08.003716120
GTCTAGA10051.559428E-57.755685120
Produced by FastQC (version 0.11.5)