FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831677

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831677
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14825752
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC169600.075.89511120
GAGCACA274750.046.18656120
AGAGCAC364950.035.436348120
AAGAGCA563300.023.078152120
GAAGAGC622500.020.948399120
GGAAGAG762500.017.72998120
CGGAAGA891950.015.239958120
TCGGAAG894850.015.088262118-119
GATCGGA1083350.012.920885120
ATCGGAA1057200.012.89215118-119
AGATCGG1188750.011.426052116-117
TAGATCG275150.011.051996120
CTAGATC70050.09.721806120
AAGATCG533300.09.469551118-119
GAGATCG361050.09.048408118-119
CACGTCT46900.08.913589120
TTAGATC121200.08.772123118-119
CACACGT41400.08.631956120
CAGATCG352750.08.193211116-117
ACACGTC50600.08.12855120
Produced by FastQC (version 0.11.5)