FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831678

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831678
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16487553
Sequences flagged as poor quality0
Sequence length53-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG63600.015.29064120
GATCTCG442900.010.544881120
TAGATCT489550.010.44745120
ATCTCGG414750.09.784261120
AGATCTC466750.09.62022120
TCGGTGG332750.09.507368120
GGTGGTC271850.09.495259120
AGTGTAG697000.09.439425120
GTAGATC653700.09.311663120
TGGTCGC220250.09.239566120
CTCGGTG349850.09.145618120
GTGGTCG235950.09.05728120
TCTCGGT385800.08.869117120
GTGTAGA673900.08.863298120
AGAGTGT828800.08.843694120
GAGTGTA754850.08.59672120
AAGAGTG943600.08.365764120
GGTCGCC211350.08.3221035120
CGGTGGT322600.08.057332120
TGTAGAT722250.07.887628120
Produced by FastQC (version 0.11.5)