FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11899492
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC172900.0110.73899120
GAGCACA298800.065.19654120
AGAGCAC413700.047.171806120
CCGATCT120900.046.0169374
TCCGATC127050.044.0808143
CGATCTA33400.042.2759255
CTTCCGA144250.038.8752061
AAGAGCA612350.032.223255118-119
GATCTAG9050.029.217956
TTCCGAT196300.029.203662
GAAGAGC702300.027.852726116-117
GGAAGAG844100.023.457169116-117
CGGAAGA981150.020.058008114-115
TCGGAAG1003150.019.758642114-115
ATCGGAA1171200.016.809017112-113
GATCGGA1192100.016.62181112-113
AGATCGG1283550.015.207621110-111
CGATCTT101250.014.2069925
CACGTCT49100.013.951863120
TAGATCG343900.013.948073112-113
Produced by FastQC (version 0.11.5)