FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15659673
Sequences flagged as poor quality0
Sequence length50-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC186700.074.503395120
GAGCACA305800.046.407234120
AGAGCAC401150.035.09624118-119
AAGAGCA622000.022.71558118-119
GAAGAGC680050.021.203222120
GGAAGAG833300.017.175081120
TCGGAAG1010300.015.041926120
CGGAAGA971850.014.917397118-119
ATCGGAA1190800.012.576729118-119
GATCGGA1203950.012.547844118-119
AGATCGG1331050.011.296749116-117
CACACGT51750.010.751523120
TAGATCG313100.010.706623116-117
ACGTCTG73300.010.608557120
CACGTCT60800.010.58449120
ACACGTC60400.09.536658118-119
CTAGATC78650.09.4524355114-115
AAGATCG501700.09.179413120
TTAGATC132000.09.14115120
GAGATCG489950.09.043821120
Produced by FastQC (version 0.11.5)