FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12265048
Sequences flagged as poor quality0
Sequence length56-126
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTAG8550.014.0418733
CTAGCCG10450.013.7766186
TCTAGCC9700.013.6148265
ATCTAGC10100.012.4812784
AGAGTGT291950.08.777354120
AAGAGTG330950.08.68595120
TAGATCT182000.08.672187120
GAGTGTA266050.08.616766120
AGTGTAG244650.08.511939120
GTGTAGA249450.08.492498120
AGATCTC192850.08.277635120
TAGGGAA483900.08.061242120
ATCTCGG194850.07.9154744120
TGTAGGG548600.07.909094120
GTGTAGG598650.07.759115120
GTAGGGA512150.07.757201120
TGTAGAT270450.07.7221193120
TCGTGTA676600.07.406256120
GTAGATC297800.07.3756557120
AAAGAGT384600.07.365072120
Produced by FastQC (version 0.11.5)