FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12028883
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC182750.0112.9244120
GAGCACA318050.066.54743120
AGAGCAC428750.048.79036118-119
AAGAGCA630300.033.60064118-119
GAAGAGC720300.029.096556116-117
GGAAGAG846550.025.017143116-117
CGGAAGA991300.021.337275114-115
TCGGAAG1036650.020.475996114-115
TCCGATC62700.019.6416133
CCGATCT60850.018.9469324
CGATCTA17350.018.1229365
ATCGGAA1195050.017.782677112-113
GATCGGA1218000.017.48374112-113
AGATCGG1320150.015.979668110-111
TAGATCG303950.014.972764110-111
CTAGATC67000.013.795202110-111
GAGATCG475900.013.40097110-111
TTAGATC109600.013.053704108-109
CAGATCG460050.012.877265110-111
CTTCCGA100350.012.6755681
Produced by FastQC (version 0.11.5)