FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831703

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831703
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17403895
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC208300.067.380486120
GAGCACA326250.043.24634120
AGAGCAC439950.033.6093120
AAGAGCA670900.022.02967120
GAAGAGC733600.020.069487118-119
GGAAGAG859450.017.368357120
TCGGAAG1030500.015.090614120
CGGAAGA1018750.014.994785120
ATCGGAA1224900.012.799649120
GATCGGA1222650.012.738424118-119
AGATCGG1360550.011.447308118-119
TAGATCG262650.010.44269116-117
TTAGATC120050.09.998734120
CTAGATC69100.09.850529110-111
AAGATCG687800.09.4332285114-115
CACACGT50800.08.976341120
ACACGTC59900.08.844116120
GAGATCG437600.08.520236120
CAGATCG395550.07.9936805116-117
TAAGATC275950.07.679417114-115
Produced by FastQC (version 0.11.5)