FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12601662
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA1583300.013.869741120
CGTATCA622800.08.593434120
TCATTAA501600.08.528693120
GCCGTAT746800.08.516321120
ATCATTA566500.08.494248120
CGCCGTA795850.08.323161120
GGTCGCC960750.08.168604120
TCGCCGT895600.08.126382120
TCCGATC77800.08.0976733
GTATCAT622650.08.04624120
CCGTATC698100.07.984516120
CATTAAA498400.07.9815273120
GTGGTCG1079150.07.8728495120
CCGATCT77050.07.8650094
TGGTCGC1004600.07.8419137120
GTCGCCG952650.07.790885120
GGTGGTC1150100.07.55933120
GGGGTAC12752.288598E-67.5293991
TCGGTGG1322150.07.4197206120
CGGTGGT1244900.07.402992120
Produced by FastQC (version 0.11.5)