FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12601662
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC181950.097.57695120
GAGCACA307050.060.53118120
AGAGCAC414800.043.861397118-119
AAGAGCA615600.030.113115120
GAAGAGC708100.025.89314118-119
GGAAGAG836400.021.94524116-117
CGGAAGA967150.019.007643116-117
TCGGAAG1003450.018.340034114-115
ATCGGAA1167800.015.807326114-115
GATCGGA1209750.015.317932112-113
CACACGT42700.014.5658045120
AGATCGG1311850.014.091916112-113
TAGATCG320100.013.352891110-111
CTAGATC93900.012.078297110-111
TTAGATC124450.011.228528120
GAGATCG501650.011.070891110-111
GCTAGAT32600.010.771264110-111
CAGATCG458200.010.683157112-113
AAGATCG395650.010.558305110-111
ACACGTC48300.010.53574120
Produced by FastQC (version 0.11.5)