FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831707

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831707
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12352617
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC91450.059.240543120
GAGCACA141200.043.96322120
AGAGCAC187600.032.487957120
AAGAGCA336300.019.614527120
GAAGAGC353450.017.979334118-119
GGAAGAG453650.015.024416120
TCGGAAG521200.013.774951120
CGGAAGA542750.013.528388120
GATCGGA598150.012.589888120
ATCGGAA617200.011.764147118-119
AGATCGG648400.011.266056120
TAGATCG192400.010.819868120
CTAGATC42900.08.988674118-119
TTAGATC89100.08.972434118-119
GAGATCG233550.08.84613118-119
AAGATCG270950.08.678005118-119
CAGATCG188100.07.4337120
CTCTAGA13051.3777386E-57.207252120
TGAGATC103450.07.0309896120
TAAGATC106600.06.4703120
Produced by FastQC (version 0.11.5)