FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11703882
Sequences flagged as poor quality0
Sequence length53-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA742750.011.325591120
TCATTAA251500.08.6124115120
GCGTCGG79650.08.361665120
CGTATCA313250.08.255468120
GTGGTCG521950.08.219259120
GGTGGTC557750.08.218818120
TGGTCGC477100.08.174468120
GTCGCCG478750.08.133761120
GCCGTAT388000.08.087709120
GGTCGCC468000.07.9231553120
CTCGGTG684750.07.8774056120
GTATCAT317250.07.678563120
CGCCGTA393950.07.6457157120
TCGGTGG653500.07.5654917120
TCTCGGT720100.07.4990325120
ATCTCGG775900.07.477842120
GATCTCG835850.07.393745120
AGATCTC852950.07.3932385120
CGGTGGT623400.07.382171120
TCGCCGT448150.07.363679120
Produced by FastQC (version 0.11.5)