FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831709

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831709
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11703882
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC140350.078.604866120
GAGCACA222450.047.481636120
AGAGCAC299350.036.97097120
AAGAGCA454800.024.966606120
GAAGAGC524650.022.070415120
GGAAGAG646850.017.902487118-119
CGGAAGA768150.015.247671120
TCGGAAG770950.015.107431116-117
ATCGGAA895300.013.028715116-117
GATCGGA912500.012.914252114-115
CACACGT50850.012.689075120
AGATCGG1010000.011.902072120
CTAGATC55100.011.583048120
TAGATCG230150.011.06328112-113
CACGTCT46700.010.212337120
TTAGATC79200.09.476577114-115
GAGATCG358300.09.258645120
ACACGTC53000.09.130747120
AAGATCG352050.09.125347112-113
CAGATCG379900.08.78761120
Produced by FastQC (version 0.11.5)