FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831710

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831710
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14071869
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG99750.012.452809120
AAAAAAA1243650.011.60451120
CGTATCA531150.09.1173120
TCATTAA424100.08.744402120
GTGGTCG937500.08.730779120
GGTCGCC825000.08.590251120
CGCCGTA700250.08.578095120
GTATCAT530000.08.559646120
CCGTATC596450.08.451137120
GCCGTAT650650.08.171394120
CATTAAA437050.08.114584120
GGTGGTC1002950.08.077266120
GTCGCCG839800.08.067295120
TGGTCGC864150.08.062189120
ATCATTA484200.08.018431120
TCGCCGT803100.07.987631120
CTCGGTG1270000.07.871929120
CGGTGGT1102250.07.7742414120
TATCATT570700.07.686336120
CCCCCCA8606.8913307E-47.6738461
Produced by FastQC (version 0.11.5)