FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831717

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831717
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12424939
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC131250.069.72566120
GAGCACA208950.044.382317120
AGAGCAC281450.034.48062120
AAGAGCA446650.023.196115120
GAAGAGC501800.019.954779118-119
GATCCTA14950.018.4787671
GGAAGAG622650.016.91831120
GATCCAC37450.015.3812131
GATCCAA29900.015.3334461
TCGGAAG744700.015.294112120
CGGAAGA736850.014.898464120
GATCCTG59900.014.5228291
GATCCTT40600.013.4639731
GATCCAT41150.013.4268561
ATCGGAA874800.013.320991120
GATCCTC46350.013.3154521
GATCGGA881650.013.0607195118-119
GATCCGG74750.012.5812881
GATCTCT36100.012.537181
GATCATA40800.012.5335871
Produced by FastQC (version 0.11.5)