FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18397842
Sequences flagged as poor quality0
Sequence length53-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGG378650.08.761974120
TAGATCT420900.08.737684120
GGTGGTC262200.08.534752120
GTGTAGA552400.08.525715120
AGATCTC425000.08.498109120
AGTGTAG561350.08.432533120
GAGTGTA608850.08.385598120
GTGGTCG233900.08.207947120
GTAGATC549200.08.138429120
CTCGGTG332300.08.070342120
TCTCGGT337600.08.032501120
TCGGTGG318000.07.9993286120
GCGTCGG113000.07.963933120
AGAGTGT659900.07.88235120
TGGTCGC215700.07.8435698120
GATCTCG432850.07.8034334120
TAGGGAA1034300.07.4594913120
AAGAGTG732200.07.439965120
GTGTAGG1230100.07.2231965120
CCTACAC57800.07.16196971
Produced by FastQC (version 0.11.5)