FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15931612
Sequences flagged as poor quality0
Sequence length51-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA3559500.016.763052120
TCATTAA1244200.010.917548120
CATTAAA1163150.010.835642120
ATCATTA1384050.010.570352120
TTAAAAA1146900.09.652223120
TATCATT1568300.09.642906120
CGTATCA1724000.09.595164120
GTATCAT1615250.09.51525120
TCTCGGG44250.09.512263120
ATTAAAA1187900.09.410194120
CCGTATC1905400.09.382268120
GCGTCGG111800.09.143369120
GCCGTAT2047200.08.95857120
CCCCCCC3100.00245943558.70944982-83
CCTAGTC3150.00284322728.57117762-63
TAAAAAA1161400.08.527287120
CGCCGTA2188500.08.322462120
TCGCCGT2384200.08.13114120
CCGATCT75050.08.0744812
TCCGATC74350.08.0698033
Produced by FastQC (version 0.11.5)