FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15931612
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC215950.0144.71043120
GAGCACA366900.082.175606120
AGAGCAC510050.060.19534118-119
AAGAGCA758200.039.784412118-119
GAAGAGC873100.035.03873116-117
GGAAGAG1050150.028.653812116-117
CGGAAGA1236400.024.933107114-115
TCGGAAG1261200.023.82565114-115
ATCGGAA1439850.021.420387112-113
GATCGGA1464750.020.614014112-113
AGATCGG1589250.019.392935110-111
CTAGATC103250.018.365396108-109
TAGATCG380500.017.668823110-111
GAGATCG581150.015.820062110-111
TTAGATC115550.015.777538108-109
CAGATCG551100.015.384605112-113
CCTAGAT31000.014.491739108-109
GCTAGAT30450.014.41039108-109
AAGATCG431650.014.401211110-111
TCTAGAT42800.013.425568108-109
Produced by FastQC (version 0.11.5)