FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831725

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831725
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17438155
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC121900.046.54599120
GAGCACA174850.030.716427120
AGAGCAC216750.027.233652120
AAGAGCA361200.017.901325120
GAAGAGC401800.017.170444120
GGAAGAG511800.013.467951120
TCGGAAG553800.012.871116120
ATCGGAA685650.011.740631120
GATCGGA666400.011.689808120
CGGAAGA629200.011.318874120
AGATCGG738100.010.9650135120
TAGATCG183200.010.132401120
TTAGATC89500.09.125729120
CTAGATC47900.08.896684116-117
AAGATCG382650.07.680836120
GAGATCG317800.07.340136120
TCTAGAT30500.06.0748286118-119
CAGATCG316150.05.910584120
CCTAGAT23300.05.433878114-115
TAAGATC151350.05.1824355118-119
Produced by FastQC (version 0.11.5)