FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16067446
Sequences flagged as poor quality0
Sequence length52-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG66000.010.093297120
TGGTCGC379750.09.7824335120
GGTCGCC356900.09.433446120
GTCGCCG353800.09.346474120
GTGGTCG418050.09.259457120
CTCGGTG589050.09.179661120
GGTGGTC464300.09.035091120
TCGCCGT349750.08.819814120
CGCCGTA307050.08.697711120
TCGGTGG558600.08.487508120
TCTCGGT625200.08.389701120
GATCTCG725000.08.360599120
ATCTCGG670800.08.248821120
CGGTGGT540900.08.177199120
TAGATCT790500.07.9867096120
AGATCTC760900.07.847829120
GCCGTAT322450.07.6681194120
CGTATCA274350.07.6562676120
AGTGTAG1039950.07.479052120
CCGTATC329600.07.319693120
Produced by FastQC (version 0.11.5)