FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14948245
Sequences flagged as poor quality0
Sequence length67-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG98450.012.013253120
TGGTCGC274550.010.714816120
TAGATCT606000.010.570655120
GGTGGTC340850.09.881201120
ATCTCGG502400.09.786882120
GTGGTCG304400.09.782439120
TCTCGGT456350.09.708863120
AGATCTC570200.09.697114120
CTCGGTG435600.09.4684515120
GATCTCG548950.09.46005120
TCGGTGG419350.09.148171120
GTGTAGA817700.08.964618120
AGTGTAG852900.08.819888120
CGGTGGT408500.08.8032875120
GTAGATC782800.08.697058120
TGTAGAT825300.08.69293120
AGAGTGT998500.08.405609120
GAGTGTA922800.08.327459120
GGTCGCC275450.08.325891120
AAGAGTG1093950.07.6612225120
Produced by FastQC (version 0.11.5)