FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14948245
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC168200.075.95228120
GAGCACA265200.049.09281120
AGAGCAC356500.036.133537118-119
AAGAGCA549400.023.723902118-119
GAAGAGC622650.020.835123118-119
GGAAGAG772700.017.490204120
TCGGAAG914900.014.745683118-119
CGGAAGA928550.014.481543120
GATCGGA1064700.012.808116120
ATCGGAA1060950.012.629665118-119
AGATCGG1170300.011.519062120
TAGATCG262100.010.834183118-119
CTAGATC67550.010.122162110-111
AAGATCG540800.09.525948114-115
TTAGATC113700.09.080017110-111
GAGATCG367050.08.824456118-119
TAAGATC215200.08.449072120
CCTAGAT24050.08.180867120
CAGATCG339600.08.042797118-119
TGAGATC162150.07.576889118-119
Produced by FastQC (version 0.11.5)