FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11925211
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC121450.065.12388120
GAGCACA187850.042.484562120
AGAGCAC244250.032.58166118-119
AAGAGCA382300.021.011442120
GAAGAGC428500.019.140045120
GGAAGAG535300.016.134106120
TCGGAAG615450.014.085716120
CGGAAGA634100.013.333611118-119
GATCGGA726350.012.122835120
ATCGGAA735650.011.744598118-119
AGATCGG810000.011.1354475120
TAGATCG207850.010.356857118-119
CTAGATC47950.010.15704118-119
ACACGTC45150.09.06094120
CACGTCT43250.09.008565120
TTAGATC70700.09.001132120
CACACGT38550.08.843607118-119
GAGATCG359700.08.701558120
GCTAGAT20150.08.057456112-113
CAGATCG306550.07.83774120
Produced by FastQC (version 0.11.5)