FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831736

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831736
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14365657
Sequences flagged as poor quality0
Sequence length51-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA812050.012.540959120
TCATTAA305850.010.261831120
CGTATCA407300.09.915904120
CCGTATC492500.09.882033120
TGGTCGC720250.09.790081120
GCCGTAT534350.09.748226120
GGTCGCC686900.09.54901120
GCGTCGG113800.09.492092120
CATTAAA312500.09.256114120
GTGGTCG784750.09.184243120
GGTGGTC862950.09.019575120
GTATCAT414550.08.931819120
GTCGCCG711300.08.841789120
CGGTGGT961750.08.748176120
CGCCGTA588550.08.634275118-119
ATCATTA363650.08.6307745120
TATCATT432650.08.5858965120
TCGGTGG1010100.08.43637120
CTCGGTG1108200.08.209425120
TCGCCGT681850.08.079519120
Produced by FastQC (version 0.11.5)