FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14365657
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC215150.081.47281120
GAGCACA343000.049.388065120
AGAGCAC451000.039.131077120
AAGAGCA676450.026.959692120
GAAGAGC757600.023.580547118-119
GGAAGAG913300.019.426151118-119
CGGAAGA1072750.016.764427116-117
TCGGAAG1093150.016.257288118-119
CACGTCT71000.015.535315120
CACACGT58500.015.160299120
ATCGGAA1289050.014.084063114-115
GATCGGA1330500.013.533524116-117
ACACGTC71850.013.380724120
ACGTCTG77050.012.961308120
AGATCGG1476100.012.28409120
TAGATCG360200.011.83502120
CTAGATC96350.010.575227118-119
TTAGATC109550.010.149617118-119
GCACACG98800.010.032551120
GAGATCG618800.09.879667118-119
Produced by FastQC (version 0.11.5)