FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15122456
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC202350.077.44587120
GAGCACA321400.048.935993120
AGAGCAC429100.036.935097120
AAGAGCA637900.025.257582120
GAAGAGC726600.022.108833118-119
GGAAGAG902500.018.608414120
CGGAAGA1059450.015.61522118-119
TCGGAAG1076200.015.474445118-119
ATCGGAA1242200.013.456702120
GATCGGA1257600.013.310032116-117
CACGTCT67650.012.186614120
CTAGATC81700.012.178641120
AGATCGG1384450.012.054603116-117
TAGATCG289600.011.54747114-115
TTAGATC118450.010.070215114-115
GAGATCG437050.010.049633120
AAGATCG583850.010.032788110-111
ACACGTC68400.09.913338118-119
CACACGT68250.09.788544120
TAAGATC215050.09.319713120
Produced by FastQC (version 0.11.5)