FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831745

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831745
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17525590
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC135400.043.184402120
GAGCACA181450.033.220963120
AGAGCAC232650.027.142454120
GAAGAGC418000.016.598225120
AAGAGCA409150.016.210703120
GGAAGAG540500.013.793619120
TCGGAAG585350.012.84856118-119
CGGAAGA645800.011.882362120
GATCGGA702600.011.693648120
ATCGGAA748400.011.028006120
AGATCGG747500.010.924532118-119
TAGATCG192100.010.448916120
CTAGATC49250.09.746018120
TTAGATC92100.08.054306116-117
CCTAGAT21400.07.864892120
AAGATCG339700.07.230085120
GAGATCG321400.07.1665697118-119
CAGATCG329400.06.509949120
CACGTCT51000.05.7447405120
TAAGATC171500.05.234089120
Produced by FastQC (version 0.11.5)