FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831747

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831747
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11376643
Sequences flagged as poor quality0
Sequence length50-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC119050.060.060493120
GAGCACA172000.040.608475120
AGAGCAC227350.031.087378118-119
AAGAGCA342950.021.72169120
GAAGAGC398700.019.530743120
GGAAGAG508150.016.53943120
TCGGAAG569100.014.477773118-119
CGGAAGA598050.013.701862120
GATCGGA671500.012.696181120
ATCGGAA670550.012.628711120
AGATCGG763000.011.882943120
CTAGATC48350.011.851808120
TAGATCG187750.010.547885114-115
CCCTAGA5751.8353603E-99.966227118-119
CCTAGAT15400.09.717067112-113
TTAGATC74250.09.346921120
AAGATCG292850.09.186234118-119
CGCTAGA6951.9880074E-49.161228120
GAGATCG278300.09.151723118-119
GGCCTAG2800.00645919059.096678110-111
Produced by FastQC (version 0.11.5)