FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831748
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15887622
Sequences flagged as poor quality0
Sequence length51-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGTG452300.09.6060295120
TAGATCT613800.09.561893120
TCGGTGG424800.09.380273120
GTGGTCG316350.09.352139120
GATCTCG574150.09.323338120
ATCTCGG523050.09.144228120
TGGTCGC284650.09.044363120
AGATCTC586700.08.980702120
GTGTAGA812050.08.890265120
GGTGGTC355500.08.879286120
TCTCGGT471150.08.817959118-119
GGTCGCC283350.08.725814120
GTAGATC783250.08.558244120
AGTGTAG846550.08.478333120
AGAGTGT988400.08.439446120
GAGTGTA908900.08.411728120
GCGTCGG84550.08.304321120
CGGTGGT418200.07.9498262120
AAGAGTG1085400.07.773694120
TGTAGAT833050.07.751274120
Produced by FastQC (version 0.11.5)