FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831750

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831750
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12585783
Sequences flagged as poor quality0
Sequence length53-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCT226200.09.813664120
GAGTGTA359500.09.696149120
AGATCTC228150.09.387929120
GTGTAGA327400.09.3457365120
AGAGTGT398400.09.2463665120
AGTGTAG327150.09.178677118-119
GCGTCGG72800.08.982912120
GATCTCG239800.08.906823120
GTAGGGA690000.08.486385120
TGTAGAT366350.08.48312120
ATCTCGG215050.08.369583120
TAGGGAA655650.08.27215120
GTGTAGG810500.08.246206120
AAGAGTG448000.08.088741120
TGGTCGC117300.07.979003120
TCTCGGT192000.07.968214120
CTCGGTG193550.07.9663973120
GTAGATC341700.07.9362526120
TGTAGGG743750.07.8488836120
CGGTGGT184450.07.676361120
Produced by FastQC (version 0.11.5)