FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12585783
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC126550.067.62742120
GAGCACA188500.043.836273120
AGAGCAC260350.032.995304120
AAGAGCA413000.021.809525120
GAAGAGC464800.019.986263120
GGAAGAG578000.016.010256118-119
TCGGAAG670200.014.789491120
CGGAAGA676500.013.783437118-119
ATCGGAA800600.012.733197120
GATCGGA794300.012.603453118-119
AGATCGG885100.011.858237120
CTAGATC56650.010.646851112-113
TAGATCG235400.010.39677118-119
TTAGATC97400.09.880103120
GAGATCG401350.09.415019120
AAGATCG286400.08.422558120
TGAGATC161900.07.786208118-119
CAGATCG298100.07.3922234116-117
CGCTAGA7903.5788435E-77.308458118-119
CCTAGAT21700.06.9473386116-117
Produced by FastQC (version 0.11.5)